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Quake ii hrp5/30/2023 ![]() ![]() Consequently, lab-on-chip devices have a high potential for biomedical research e.g., to perform immunological assays to cultivate cells and to construct organs-on-a-chip for toxicological studies and disease modeling as well as personalized medicine. As these “labs-on-chips” allow a high integration density of multiple functions such as mixing and separation of small amounts of fluids, even complex samples can be prepared, processed, and analyzed in a short time. Stimulated by advancements in the fabrication of miniaturized microfluidic devices and the miniaturization of biochemical analysis, microfluidic systems have evolved to a powerful method in biomedical and chemical research over the last decades. The functionality of the microvalves was shown in four hourly switching cycles and visualized by the path-dependent substrate conversion. Consequently, either the enzyme cascade reaction GOx-HRP or GOx-Myo was performed and continuously quantified by ultraviolet-visible (UV-Vis) spectroscopy. Switching of the microvalves was achieved within 4 to 6 s and thereby the fluid pathway of the enzyme substrate solution (5 mmol/L) in the device was determined. As a proof-of-principle, the biocatalysts glucose oxidase (GOx), horseradish peroxidase (HRP) and myoglobin (Myo) were immobilized in photopatterned hydrogel dot arrays (diameter of the dots: 350 µm, amount of enzymes: 0.13–2.3 µg) within three compartments of the device. To fill this gap, we show the integration of thermoresponsive poly( N-isopropylacrylamide) (PNiPAAm) microvalves (diameter: 500 and 600 µm) into PDMS-on-glass microfluidic devices for the control of parallelized enzyme-catalyzed cascade reactions. However, an application of this valve concept for the control of multistep reactions was not yet shown. To achieve flow control in microfluidics, stimuli-responsive hydrogel microvalves were previously introduced. Compartmentalized microfluidic devices with immobilized catalysts are a valuable tool for overcoming the incompatibility challenge in (bio) catalytic cascade reactions and high-throughput screening of multiple reaction parameters. ![]()
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